Here are some general recommended settings (no matter the board) you can use if you have some problems with booting your system or the card is not detected, and so on:
For ASUS B250 Mining Expert, PCIe Link Speed needs to be set in two different places:
Advanced\PCH Configuration\PCI Express Configuration -> PCIe Speed -> Gen 2
Advanced\System Agent (SA) Configuration\PEG Port Configuration -> PCIEX16_1 Link Speed -> Gen 2
And here a few additional tips on B250: