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AsRock H110 pro btc 16x slot problem

Hello guys, I have an asrock h110 with 12 1660super working perfectly for 6 months now all card are connected by 1x pcie slot I tried to add the 13th card on the 16x slot but gpu doesn’t show up in hive.

Is not a gpu/riser problem 100% cause at moment i mine with that card on another rig.

Any suggestion?

So if you remove one of the other cards the 16x slot card works fine?